BCG vaccine should be stored in a refrigerator (fridge) between 2 and 8 °C and must not be frozen. Keep it and the diluent on the middle shelf. It must also be kept out of direct sunlight. To prepare the vaccine for administration the vial of diluent should be added to the vial of dried vaccine. Do not use alcohol or ether to clean the top of the vial as it may kill the BCG. After making up the vaccine it will last for 6 hours if kept in a refrigerator or cool box.